Description
This Wine Spoilage PCR Micro Test Kit from Microbiologique is designed to simultaneously detect yeasts and bacteria using one test within two hours when tested directly from a wine sample or 24-48 hours if using enrichment media/steps.
Kit Components
- Lysis Solution (100 µL)
- Taq Beads (3 strips of 8 tubes)
- PCR Buffer (1.5 mL)
- Lateral Flow Strips (24 strips)
- Detection Reagent (4 mL)
- Resuspension Solution (30mL)
Principle of the Test
The Wine Spoilage Micro Test consists of two steps:
- Concentration by centrifugation or enrichment using media that enhances the growth of the target spoilage organisms
- Detection of the target spoilage organisms by polymerase chain reaction (PCR).
Primer probes unique to each spoilage organism are multiplexed and amplify the target genes. Application of the amplified product on a Lateral Flow (LF) strip results in labeling of the amplicons with gold nanoparticles and subsequent hybridization of the labeled amplicons to complementary regions on the strip. In addition, the test strip contains a control line (IAC – Internal Amplification Control) to indicate the validity of both the PCR amplification and lateral flow detection.
Key Features
- Detects wine spoilage yeasts (Zygosaccharomyces, Brettanomyces, and Saccharomyces) and bacteria (Acetobacter, Gluconobacter, Lactobacillus, Oenococcus, Pediococcus and acetic acid bacteria) using a single test
- Direct detection from a wine sample in under 2 hours
- Detection from enrichment: 24 – 48 hours
- Limit of Detection: 1 cfu/sample
